453

U. S. Department of Agriculture,
Bureau of Animal Industry.

Washington, D. C. 1886
2 B.

replies to the following questions in order that I may not only
avoid doing you injustice but that I may have the material at hand
to do you justice. I am the more interested in this because we
have lately made substantial progress as we believe in the study
of this disease and in developing a method of prevention.

1. What were the nature and characters of the germ contained
in the Pasteur vaccine?

2. Did you make any cultures of this, and if so, in what me-
dia?

3. If this germ was a fine bacillus, as stated by the German
investigators, please state what characters were replied upon in the
investigations of the germs found in American swine plague to dis-
tinguish between Pasteur's bacillus and the bacillus of mouse sep-
ticaemia, so called, which is sometimes found as a septic or con-
taminating germ?

4. In what liquids or tissues, from animals affected with
American swine plague, did you find the germs indentical with those
in Pasteur's vaccine, and did they exist pure and uncontaminated
with other germs?

5. Were an cultivations made of the germs found in American
swine plague, and if so, by what methods and in what media?