454

U. S. Department of Agriculture,
Bureau of Animal Industry.

Washignton, D. C., 1886.

3 B.

6. Were any inoculation experiments made with pure cultures
of such germs to determine which were pathogenic?

7. If the germs found in Pasteur's vaccine are identical with
the germs which cause American swine plague, and a mild attack was
produced in your experimental animals by this vaccine, how do you
account for the fact that your animals were unable to resist exposure
to the contagion of American swine plague, seeing that such
mild attacks unquestionably confer immunity against the rouget of
France?

8. Do you authorize and endorse the statements by D. Gerth
referred to above, viz., (1) that microbes indentical with those
in Pasteur's vaccine exist in American swine plague; (2) that American
swine plague is caused by this same germ; (3) that Pasteur's
vaccine does not confer immunity from the disease for which it was
intended; and (4) that inoculation for swine plague is consequently
a failure?

9. Can you furnish me a preparation for microscopical examination
which shows the germs found in the swine plague of Nebraska?

I trust you will appreciate my reasons for desiring information
on this subject and that you may find time to give me an early reply,
even though it be brief.